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KMID : 1098420220300040278
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Korean Journal of Medicinal Crop Science
2022 Volume.30 No. 4 p.278 ~ p.286
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Kaempferol-3-O-glucoside Isolated from Lonicera japonica Protects Neuronal HT22 Cells against Glutamate-Induced Oxidative Stress
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Ma Choong-Je
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Abstract
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Background: In this study, we aim to test the neuroprotective activity of kaempferol-3-O-glucoside in HT22 cells originating from mice hippocam against glutamate induced oxidative stress and to elucidate the mechanisms of action of this neuroprotective activity.
Methods and Results: Pretreatment of HT22 cells with kaempferol-3-O-glucoside increased cell viability after exposure to glutamate induced oxidative stress. Kaempferol-3-O-glucoside decreased reactive oxygen species and intracellular Ca2+ concentration, which increased with glutamate induced oxidative stress. Moreover, kaempferol-3-O-glucoside maintained the mitochondrial membrane potential at control levels, increased glutathione reductase and glutathione peroxidase levels, and synthesized high levels of the endogenous antioxidant glutathione.
Conclusions: Overall, these results suggest that kaempferol-3-O-glucoside isolated from Lonicera japonica exerted a potent neuroprotective activity in glutamate injured HT22 cells through the antioxidative pathway and can be potentially used for the treatment or prevention of Alzheimer's disease.
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KEYWORD
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Lonicera japonica, Alzheimer's Disease, Antioxidant, Kaempferol-3-O-glucoside, Neuroprotection
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